Publication
Representative publications
Chemical biology, chemoproteomics, therapeutic covalent ligands
1. Koo, T.-Y.,† Lai, H.,† Nomura, D. K., & Chung, C. Y.-S.* (2023). N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nature Communications, 14(1), 3564. († These authors contributed equally)
2. Chung, C. Y.-S.,† Shin, H. R.,† Berdan, C. A., Ford, B., Ward, C. C., Olzmann, J. A., Zoncu, R.,* & Nomura, D. K.* (2019). Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nature Chemical Biology, 15(8), 776–785. († These authors contributed equally; * co-corresponding authors)
** Featured in News and Views in Nature Chemical Biology, 2019, 15(8), 760.
Redox biology and molecular probes
1. Lai, H., & Chung, C. Y.-S.* (2024). Superoxide-responsive quinone methide precursors (QMP-SOs) to study superoxide biology by proximity labeling and chemoproteomics. RSC Chemical Biology. https://doi.org/10.1039/D4CB00111G
2. Lee, S.,† Chung, C. Y.-S.,† Liu, P., Craciun, L., Nishikawa, Y., Bruemmer, K. J., Hamachi, I., Saijo, K., Miller, E. W., & Chang, C. J. (2020). Activity-Based Sensing with a Metal-Directed Acyl Imidazole Strategy Reveals Cell Type-Dependent Pools of Labile Brain Copper. Journal of the American Chemical Society, 142(35), 14993–15003. († These authors contributed equally)
3. Chung, C. Y.-S., Posimo, J. M., Lee, S., Tsang, T., Davis, J. M., Brady, D. C., & Chang, C. J. (2019). Activity-based ratiometric FRET probe reveals oncogene-driven changes in labile copper pools induced by altered glutathione metabolism. Proceedings of the National Academy of Sciences, 116(37), 18285.
4. Chung, C. Y. S., Timblin, G. A., Saijo, K., & Chang, C. J. (2018). Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining. Journal of the American Chemical Society, 140(19), 6109–6121.
Cancers
1. Husain, A., Chiu, Y.-T., Sze, K. M.-F., Ho, D. W.-H., Tsui, Y.-M., Suarez, E. M. S., Zhang, V. X., Chan, L.-K., Lee, E., Lee, J. M.-F., Cheung, T.-T., Wong, C. C.-L., Chung, C. Y.-S., & Ng, I. O.-L. (2022). Ephrin-A3/EphA2 axis regulates cellular metabolic plasticity to enhance cancer stemness in hypoxic hepatocellular carcinoma. Journal of Hepatology, 77(2), 383–396.
2. Chan, K. K.-S., Au, K.-Y., Suen, L.-H., Leung, B., Wong, C.-Y., Leow, W.-Q., Lim, T. K.-H., Ng, I. O.-L., Chung, C. Y.-S., & Lo, R. C.-L. (2023). Sortilin-Driven Cancer Secretome Enhances Tumorigenic Properties of Hepatocellular Carcinoma via de Novo Lipogenesis. American Journal of Pathology, 193(12), 2156–2171.
3. Huang, H., Tsui, Y.-M., Ho, D. W.-H., Chung, C. Y.-S., Sze, K. M.-F., Lee, E., Cheung, G. C.-H., Zhang, V. X., Wang, X., Lyu, X., & Ng, I. O.-L. (2024). LANCL1, a cell surface protein, promotes liver tumor initiation through FAM49B-Rac1 axis to suppress oxidative stress. Hepatology, 79(2), 323-340.
4. Bao, M. H.-R., Yang, C., Tse, A. P.-W., Wei, L., Lee, D., Zhang, M. S., Goh, C. C., Chiu, D. K.-C., Yuen, V. W.-H., Law, C.-T., Chin, W.-C., Chui, N. N.-Q., Wong, B. P.-Y., Chan, C. Y.-K., Ng, I. O.-L., Chung, C. Y.-S., Wong, C.-M., & Wong, C. C.-L. (2021). Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer. Cell Reports, 34(4).
5. Mao, X., Tey, S. K., Yeung, C. L. S., Kwong, E. M. L., Fung, Y. M. E., Chung, C. Y. S., Mak, L.-Y., Wong, D. K. H., Yuen, M.-F., Ho, J. C. M., Pang, H., Wong, M. P., Leung, C. O.-N., Lee, T. K. W., Ma, V., Cho, W. C.-S., Cao, P., Xu, X., Gao, Y., & Yam, J. W. P. (2020). Nidogen 1-Enriched Extracellular Vesicles Facilitate Extrahepatic Metastasis of Liver Cancer by Activating Pulmonary Fibroblasts to Secrete Tumor Necrosis Factor Receptor 1. Advanced Science, 7(21), 2002157.
Full Publication List
36. Lai, H., & Chung, C. Y.-S.* (2024). Superoxide-responsive quinone methide precursors (QMP-SOs) to study superoxide biology by proximity labeling and chemoproteomics. RSC Chemical Biology, 5(9), 924–937. https://doi.org/10.1039/D4CB00111G
**In the themed collection: 2024 RSC Chemical Biology Emerging Investigators**
35. Lam, Y.-Y., Lee, N.-S., Lee, S.-F., & Chung, C. Y.-S.* (2023). Organometallic d6, d8 and d10 metal complexes for biosensing and imaging, cancer therapy and theranosis. Journal of Organometallic Chemistry, 122982. https://doi.org/10.1016/j.jorganchem.2023.122982 35. Lam, Y.-Y., Lee, N.-S., Lee, S.-F., & Chung, C. Y.-S.* (2023). Organometallic d6, d8 and d10 metal complexes for biosensing and imaging, cancer therapy and theranosis. Journal of Organometallic Chemistry, 122982. https://doi.org/10.1016/j.jorganchem.2023.122982
34. Chan, K. K.-S., Au, K.-Y., Suen, L.-H., Leung, B., Wong, C.-Y., Leow, W.-Q., Lim, T. K.-H., Ng, I. O.-L., Chung, C. Y.-S., & Lo, R. C.-L. (2023). Sortilin-Driven Cancer Secretome Enhances Tumorigenic Properties of Hepatocellular Carcinoma via de Novo Lipogenesis. American Journal of Pathology, 193(12), 2156–2171. https://doi.org/10.1016/j.ajpath.2023.08.005
33. Huang, H., Tsui, Y. M., Ho, D. W., Chung, C. Y.-S., Sze, K. M., Lee, E., Cheung, G. C., Zhang, V. X., Wang, X., Lyu, X., & Ng, I. O. (2024). LANCL1, a cell surface protein, promotes liver tumor initiation through FAM49B-Rac1 axis to suppress oxidative stress. Hepatology (Baltimore, Md.), 79(2), 323–340. https://doi.org/10.1097/HEP.0000000000000523
32. Koo, T.-Y., Lai, H., Nomura, D. K., & Chung, C. Y.-S.* (2023). N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nature Communications, 14(1), 3564. https://doi.org/10.1038/s41467-023-39268-w
31. Hoshi, K., Messina, M. S., Ohata, J., Chung, C. Y.-S., & Chang, C. J. (2022). A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues. Nature Protocols. https://doi.org/10.1038/s41596-022-00694-7
30. Tey, S. K., Wong, S. W. K., Yeung, C. L. S., Li, J. Y. K., Mao, X., Chung, C. Y.-S., & Yam, J. W. P. (2022). Liver cancer cells with nuclear MET overexpression release translation regulatory protein-enriched extracellular vesicles exhibit metastasis promoting activity. Journal of Extracellular Biology, 1(5), e39. https://doi.org/10.1002/jex2.39
29. Husain, A., Chiu, Y.-T., Sze, K. M.-F., Ho, D. W.-H., Tsui, Y.-M., Suarez, E. M. S., Zhang, V. X., Chan, L.-K., Lee, E., Lee, J. M.-F., Cheung, T.-T., Wong, C. C.-L., Chung, C. Y.-S., & Ng, I. O.-L. (2022). Ephrin-A3/EphA2 axis regulates cellular metabolic plasticity to enhance cancer stemness in hypoxic hepatocellular carcinoma. Journal of Hepatology. https://doi.org/10.1016/j.jhep.2022.02.018
28. Bao, M. H.-R., Yang, C., Tse, A. P.-W., Wei, L., Lee, D., Zhang, M. S., Goh, C. C., Chiu, D. K.-C., Yuen, V. W.-H., Law, C.-T., Chin, W.-C., Chui, N. N.-Q., Wong, B. P.-Y., Chan, C. Y.-K., Ng, I. O.-L., Chung, C. Y.-S., Wong, C.-M., & Wong, C. C.-L. (2021). Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer. Cell Reports, 34(4). https://doi.org/10.1016/j.celrep.2020.108676
27. Mao, X., Tey, S. K., Yeung, C. L. S., Kwong, E. M. L., Fung, Y. M. E., Chung, C. Y. S., Mak, L.-Y., Wong, D. K. H., Yuen, M.-F., Ho, J. C. M., Pang, H., Wong, M. P., Leung, C. O.-N., Lee, T. K. W., Ma, V., Cho, W. C.-S., Cao, P., Xu, X., Gao, Y., & Yam, J. W. P. (2020). Nidogen 1-Enriched Extracellular Vesicles Facilitate Extrahepatic Metastasis of Liver Cancer by Activating Pulmonary Fibroblasts to Secrete Tumor Necrosis Factor Receptor 1. Advanced Science, 7(21), 2002157. https://doi.org/10.1002/advs.202002157
26. Lee, S.,† Chung, C. Y.-S.,† Liu, P., Craciun, L., Nishikawa, Y., Bruemmer, K. J., Hamachi, I., Saijo, K., Miller, E. W., & Chang, C. J. (2020). Activity-Based Sensing with a Metal-Directed Acyl Imidazole Strategy Reveals Cell Type-Dependent Pools of Labile Brain Copper. Journal of the American Chemical Society, 142(35), 14993–15003. https://doi.org/10.1021/jacs.0c05727 (†These authors contributed equally)
Publications before starting independent research
25. Tang, F. K., Yu, Z. H., Wong, T. H.-F., Chung, C. Y.-S., Hirao, H., & Au-Yeung, H. Y. (2020). Fluorescein-Containing Superoxide Probes with a Modular Copper-Based Trigger. ChemPlusChem, 85(4), 653–658. https://doi.org/10.1002/cplu.202000059
24. Chung, C. Y.-S.,† Posimo, J. M.,† Lee, S.,† Tsang, T.,† Davis, J. M., Brady, D. C.,# & Chang, C. J.# (2019). Activity-based ratiometric FRET probe reveals oncogene-driven changes in labile copper pools induced by altered glutathione metabolism. Proceedings of the National Academy of Sciences, 116(37), 18285. https://doi.org/10.1073/pnas.1904610116 (†These authors contributed equally; #co-corresponding authorship)
23. Chung, C. Y.-S.,† Shin, H. R.,† Berdan, C. A., Ford, B., Ward, C. C., Olzmann, J. A., Zoncu, R.,# & Nomura, D. K.# (2019). Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nature Chemical Biology, 15(8), 776–785. https://doi.org/10.1038/s41589-019-0308-4 (†These authors contributed equally; #co-corresponding authorship)
**Featured in News and Views in Nat. Chem. Biol., 2019, 15, 760.
22. Ward, C. C., Kleinman, J. I., Brittain, S. M., Lee, P. S., Chung, C. Y.-S., Kim, K., Petri, Y., Thomas, J. R., Tallarico, J. A., McKenna, J. M., Schirle, M., & Nomura, D. K. (2019). Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chemical Biology, 14(11), 2430–2440. https://doi.org/10.1021/acschembio.8b01083
21. Chung, C.Y.-S., Timblin, G. A., Saijo, K., & Chang, C. J. (2018). Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining. Journal of the American Chemical Society, 140(19), 6109–6121. https://doi.org/10.1021/jacs.8b02279
20. Yu, Z. H.,† Chung, C. Y.-S.,† Tang, F. K.,† Brewer, T. F., & Au-Yeung, H. Y. (2017). A modular trigger for the development of selective superoxide probes. Chemical Communications, 53(72), 10042–10045. https://doi.org/10.1039/C7CC05405J († These authors contributed equally)
19. Siu, S. K.-L., Chung, C. Y.-S., & Yam, V. W.-W. (2017). Amphiphilic oligo(ethylene glycol)- and poly(ethyleneoxide)-block-poly(propylene oxide)-block-poly-(ethylene oxide)-containing cyclometalated alkynylgold(III) complexes: From basic photophysics to self-assembly and stimuli-responsive properties. Journal of Organometallic Chemistry, 845, 177–188. https://doi.org/10.1016/j.jorganchem.2017.04.035
18. Cheng, H.-K., Chung, C. Y.-S., Zhang, K., & Yam, V. W.-W. (2017). Simple and Versatile Preparation of Luminescent Amphiphilic Platinum(II)-containing Polystyrene Complexes With Transformable Nanostructures Assisted by Pt⋅⋅⋅Pt and π–π Interactions. Chemistry – An Asian Journal, 12(13), 1509–1516. https://doi.org/10.1002/asia.201700123
17. Chung, C. Y.-S., Fung, S.-K., Tong, K.-C., Wan, P.-K., Lok, C.-N., Huang, Y., Chen, T., & Che, C.-M. (2017). A multi-functional PEGylated gold(iii) compound: Potent anti-cancer properties and self-assembly into nanostructures for drug co-delivery. Chemical Science, 8(3), 1942–1953. https://doi.org/10.1039/C6SC03210A
16. Fong, T. T.-H., Lok, C.-N., Chung, C. Y.-S., Fung, Y.-M. E., Chow, P.-K., Wan, P.-K., & Che, C.-M. (2016). Cyclometalated Palladium(II) N-Heterocyclic Carbene Complexes: Anticancer Agents for Potent In Vitro Cytotoxicity and In Vivo Tumor Growth Suppression. Angewandte Chemie International Edition, 55(39), 11935–11939. https://doi.org/10.1002/anie.201602814
15. Chow, P.-K., Cheng, G., Tong, G. S. M., Ma, C., Kwok, W.-M., Ang, W.-H., Chung, C. Y.-S., Yang, C., Wang, F., & Che, C.-M. (2016). Highly luminescent palladium(ii) complexes with sub-millisecond blue to green phosphorescent excited states. Photocatalysis and highly efficient PSF-OLEDs. Chemical Science, 7(9), 6083–6098. https://doi.org/10.1039/C6SC00462H
14. Chung, C. Y.-S., Li, S. P.-Y., Lo, K. K.-W., & Yam, V. W.-W. (2016). Synthesis and Electrochemical, Photophysical, and Self-Assembly Studies on Water-Soluble pH-Responsive Alkynylplatinum(II) Terpyridine Complexes. Inorganic Chemistry, 55(9), 4650–4663. https://doi.org/10.1021/acs.inorgchem.6b00513
13. Leung, F. C.-M., Leung, S. Y.-L., Chung, C. Y.-S., & Yam, V. W.-W. (2016). Metal–Metal and π–π Interactions Directed End-to-End Assembly of Gold Nanorods. Journal of the American Chemical Society, 138(9), 2989–2992. https://doi.org/10.1021/jacs.6b01382
12. Yang, C., Mehmood, F., Lam, T. L., Chan, S. L.-F., Wu, Y., Yeung, C.-S., Guan, X., Li, K., Chung, C. Y.-S., Zhou, C.-Y., Zou, T., & Che, C.-M. (2016). Stable luminescent iridium(iii) complexes with bis(N-heterocyclic carbene) ligands: Photo-stability, excited state properties, visible-light-driven radical cyclization and CO2 reduction, and cellular imaging. Chemical Science, 7(5), 3123–3136. https://doi.org/10.1039/C5SC04458H
11. Chan, K., Chung, C. Y.-S., & Yam, V. W.-W. (2016). Parallel folding topology-selective label-free detection and monitoring of conformational and topological changes of different G-quadruplex DNAs by emission spectral changes via FRET of mPPE-Ala–Pt(ii) complex ensemble. Chemical Science, 7(4), 2842–2855. https://doi.org/10.1039/C5SC04563K
10. Chan, K., Chung, C. Y.-S., & Yam, V. W.-W. (2015). Conjugated Polyelectrolyte-Induced Self-Assembly of Alkynylplatinum(II) 2,6-Bis(benzimidazol-2′-yl)pyridine Complexes. Chemistry – A European Journal, 21(46), 16434–16447. https://doi.org/10.1002/chem.201501804
9. Chung, C. Y.-S., Tamaru, S., Shinkai, S., & Yam, V. W.-W. (2015). Supramolecular Assembly of Achiral Alkynylplatinum(II) Complexes and Carboxylic β-1,3-Glucan into Different Helical Handedness Stabilized by Pt⋅⋅⋅Pt and/or π–π Interactions. Chemistry – A European Journal, 21(14), 5447–5458. https://doi.org/10.1002/chem.201405035
8. Chung, C. Y.-S., & Yam, V. W.-W. (2014). Induced Self-Assembly of Platinum(II) Alkynyl Complexes through Specific Interactions between Citrate and Guanidinium for Proof-of-Principle Detection of Citrate and an Assay of Citrate Lyase. Chemistry – A European Journal, 20(40), 13016–13027. https://doi.org/10.1002/chem.201402137
7. Chung, C. Y.-S., & Yam, V. W.-W. (2013). Dual pH- and Temperature-Responsive Metallosupramolecular Block Copolymers with Tunable Critical Micelle Temperature by Modulation of the Self-Assembly of NIR-Emissive Alkynylplatinum(II) Complexes Induced by Changes in Hydrophilicity and Electrostatic Effects. Chemistry – A European Journal, 19(39), 13182–13192. https://doi.org/10.1002/chem.201301547
6. Chung, C. Y.-S., Li, S. P.-Y., Louie, M.-W., Lo, K. K.-W., & Yam, V. W.-W. (2013). Induced self-assembly and disassembly of water-soluble alkynylplatinum(ii) terpyridyl complexes with “switchable” near-infrared (NIR) emission modulated by metal–metal interactions over physiological pH: demonstration of pH-responsive NIR luminescent probes in cell-imaging studies. Chemical Science, 4(6), 2453–2462. https://doi.org/10.1039/C3SC50196E
5. Chung, C. Y.-S., & Yam, V. W.-W. (2013). Selective label-free detection of G-quadruplex structure of human telomere by emission spectral changes in visible-and-NIR region under physiological condition through the FRET of a two-component PPE-SO3−–Pt(ii) complex ensemble with Pt⋯Pt, electrostatic and π–π interactions. Chemical Science, 4(1), 377–387. https://doi.org/10.1039/C2SC20897K
4. Chung, C. Y.-S., & Yam, V. W.-W. (2011). Induced Self-Assembly and Förster Resonance Energy Transfer Studies of Alkynylplatinum(II) Terpyridine Complex Through Interaction With Water-Soluble Poly(phenylene ethynylene sulfonate) and the Proof-of-Principle Demonstration of this Two-Component Ensemble for Selective Label-Free Detection of Human Serum Albumin. Journal of the American Chemical Society, 133(46), 18775–18784. https://doi.org/10.1021/ja205996e
3. Hu, Y., Chan, K. H.-Y., Chung, C. Y.-S., & Yam, V. W.-W. (2011). Reversible thermo-responsive luminescent metallo-supramolecular triblock copolymers based on platinum(ii) terpyridyl chromophores with unusual aggregation behaviour and red-near-infrared (NIR) emission upon heating. Dalton Transactions, 40(45), 12228–12234. https://doi.org/10.1039/C1DT10741K
2. Chung, C. Y.-S., Chan, K. H.-Y., & Yam, V. W.-W. (2011). “Proof-of-principle” concept for label-free detection of glucose and α-glucosidase activity through the electrostatic assembly of alkynylplatinum(ii) terpyridyl complexes. Chemical Communications, 47(7), 2000–2002. https://doi.org/10.1039/C0CC04437G
1. Yam, V. W.-W., Hu, Y., Chan, K. H.-Y., & Chung, C. Y.-S. (2009). Reversible pH- and solvent-responsive micelle-mediated self-assembly of platinum(ii) terpyridyl-based metallo-supramolecular diblock copolymers. Chemical Communications, 41, 6216–6218. https://doi.org/10.1039/B911657E